Korean Institute of Surface Engineering

pISSN : 1225-8024 | eISSN : 3399-8403


공학

한국표면공학회지 (37권2호 76-79)

Effect of Solvent on the Surface of Protein Chip Plate

단백질 칩 기판의 표면에 미치는 용매 효과

현준원;윤미영;안상민;노승정;허영덕;박헌용;송예신;피재호;김경례;

단국대학교 자연과학부;단국대학교 자연과학;(주)휴시피;

Abstract

Nickel chloride coated protein chip plate was developed by using a spin coating method. The ability of histidine tagged protein adsorption was investigated at various solvents. The surface of plate has a large aggregated nickel complex with high density in water. However, the surface of plate has a very small size of aggregated nickel complex with low density in isopropanol. The ability of protein adsorption decreased as increasing the size of alkyl chain in various alcohol solvents. The mechanism on the ability of protein adsorption at the plate surface is discussed.

Keywords

Protein chip plate;Sol-gel methods;NiCl$_2$ Film;His-tagged;